Detecting fa leptin receptor mutation in Zucker rats with tetra-primer amplification-refractory mutation system (ARMS)-PCR

Due to the genetic mutation (fa) in gene encoding for leptin receptor, homozygous Zucker rats (fa−/−) develop excessive adiposity and become an experimental animal model obesity metabolic-related diseases research. Based on tetra-primer amplification refractory system-polymerase chain reaction (ARMS-PCR), we developed a method quickly genotype with mutated fa allele from their wildtype littermates. The three genotypes are clearly discriminated 2.0% agarose gel. Our can be used as reliable tool set up maintain breeding colony facilities well assign animals control treatment groups based studies.